Yeast 2μm DNA: Its Structure and Replication

DNA Replication in Yeast

The details of chromosome replication are better understood in the budding yeast, Saccharomyces cerevisiae, than in any other eukaryotic organism. cis-Acting replicator sequences required for chromosomal replication origin function were identified on the basis of their ability to promote the extrachromosomal maintenance of plasmids. These autonomously replicating sequence (ARS) elements have be...

Structure and Replication of Yeast Prions

nants, Wickner (1994) has suggested that Sup35p and Although prion-like phenomena in yeast were discovUre2p may use a prion-like autocatalytic mechanism to ered fairly recently, the remarkably rapid progress in switch into an alternative conformational and functional their studies promises that they may become a helpful state, resulting in [PSI1] or [URE3] phenotypes. This model for the underst...

Dynamics of DNA replication in yeast.

We present a mathematical model for the spatial dynamics of DNA replication. Using this model we determine the probability distribution for the time at which each chromosomal position is replicated. From this we show, contrary to previous reports, that mean replication time curves cannot be used to directly determine origin parameters. We demonstrate that the stochastic nature of replication dy...

Structure of the SSB-DNA polymerase III interface and its role in DNA replication.

Interactions between single-stranded DNA-binding proteins (SSBs) and the DNA replication machinery are found in all organisms, but the roles of these contacts remain poorly defined. In Escherichia coli, SSB's association with the χ subunit of the DNA polymerase III holoenzyme has been proposed to confer stability to the replisome and to aid delivery of primers to the lagging-strand DNA polymera...

Role of yeast DNA polymerase epsilon during DNA replication